resolution

Super resolution microscopy techniques have revolutionized the field of biological imaging by allowing researchers to visualize structures and processes at resolutions beyond the diffraction limit of conventional light microscopy. These techniques can be broadly categorized into two groups: super-resolved ensemble microscopy techniques and super-resolved single fluorophore microscopy techniques. Super-Resolved Ensemble Microscopy Techniques Stimulated Emission Depletion … Read more

Super-resolution microscopy is a revolutionary technique that has transformed the field of biological imaging, allowing researchers to visualize structures and processes at the nanoscale with unprecedented detail. By breaking the diffraction limit of conventional light microscopy, this powerful tool has opened up new avenues for understanding the intricate workings of cells, tissues, and even entire … Read more

Summary Ultra high resolution microscopy is a powerful tool that enables the imaging of three-dimensional (3D) structures within entire mammalian cells at a resolution of 10-20 nanometers (nm). This is achieved through the use of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), which combines refined hardware and advanced data analysis techniques to image cells up to … Read more