RNA Replication Enzymes: Detailed Facts

RNA are referred to as ribonucleic acid and are the molecule same to that of DNA. Just unlike them RNA has a single strand.

RNA polymerase is a RNA dependent enzyme used up for the RNA replication. It is also called as RNA replicase and is RNA replication enzymes. It helps in catalyzing the process of getting the RNA replicated from a template of RNA.

Mostly it helps in getting the process of synthesis of the single strand of DNA fast and complementary to that of a given template of RNA. Thus it is totally different from that of the RNA polymerase that is dependent on DNA. This enzyme helps an organism to make the use of catalyst for getting the transcription of RNA done from a DNA template.

RNA is strand which has a backbone and is made up of ribose which is a sugar and is placed alternately and also has phosphate groups attached with it. It also has itself linked with sugar along with the four bases of nitrogen called the adenine, cytosine, guanine and uracil. It can also be referred to be a molecule which is polymeric.

RNA is a nucleic acid and so is DNA. Just like the DNA, RNA also has been joined as a chain with its constituents being nucleotides but RNA is single stranded and seen in nature to fold onto itself not being double. Cellular living beings use messenger RNA to get itself pass the genetic code and also helps in directing the synthesis of few proteins.

Some of the molecules of RNA play a good role inside the cell by getting the biological process catalyzed with having the gene expression under control. It also helps in communicating several responses and sensing the responses to have the signals of the cell activated. One of the processes it is involved in is protein synthesis and also getting the ribosomes protein synthesized.

Some RNA molecules play a good role in the cell by catalyzing biological processes by controlling gene expression. It is also useful for communicating multiple responses, detecting the response and activating the cell’s signal. One of the processes involved is protein synthesis and ribosomal protein synthesis.

RNA replication

The replication of the genome is essential for the continuity of life. The molecular mechanism is very similar in all groups of organisms.

Many scientists consider RNA to be the first replicated macromolecule. Like DNA, RNA can, in principle, create a negative blueprint by spontaneous base pairing. Unlike double-stranded DNA, RNA can take a variety of spatial forms and therefore also functions as an enzyme that catalyzes its own replication.

Many bacteria divide once every thirty minutes, others replicate even faster. Eukaryotic cells only replicate their genome when new cells have to be created. This happens as a result of external signals, for example tissue loss or inflammation. The life cycle of eukaryhttps://lambdageeks.com/eukaryotic-cells-vs-bacterial-cells/otic cells underlies an accurately defined sequence of activities that can be divided into different phases.

However, the situation is different at the end of the chromosome, where the lack of base prevents the replication fork from proceeding. At the point on the lagging strand where the DNA primase places the last RNA primer, the DNA polymerase will not be able to continue replication. The terminal DNA segment cannot replicate. DNA strands become shorter and shorter as the number of cell divisions increases.

To protect themselves against the rapid shortening of the DNA, eukaryotic chromosomes possess sequence repeats (telomeres) at their extremities, which do not code for proteins. Since the telomeres do not contain any vita;l information, the key parts of the DNA are protected. The telomeres get shorter each time a cell divides. The length of these so-called telomere caps defines the number of possible divisions and hence the lifespan of a cell. 

rna replication enzymes
Image credit-
RNA replication Wikipedia

RNA replication enzymes in eukaryotes

Eukaryotes are the organism that has a cell and the cell is enclosed inside a nuclear envelope. They also have other organelles with membrane.

At the level of the eukaryotes, the replication fork there is a polymerase seen for replication which is three in number and is a complex that helps the DNA get itself replicated. The main RNA replication enzyme is said to be RNA polymerase.

The enzyme of RNA polymerase helps in getting the RNA synthesized by pointing a strand of DNA. This enzyme is also the one to play a role in copying of the DNA and its sequence into a sequence of RNA at the time of transcription. The process of RNA replication in the eukaryotes takes place in the nucleus.

Image credit-

The strand of DNA is made to undergo the process of replication and then transcription within the nucleus and the proteins are then made inside the cytoplasm. RNA thus is made to travel across the membrane of the nucleus which also surrounds it before the time it goes into translation. On addition to RNA polymerase there can also be the presence of other small materials for it.

On addition to the RNA polymerase, there is α-primase and two of the DNA polymerases called the δ and ε that are needed for the process of the strand of DNA to get replicated. The δ polymerase is the major or basic polymerase inside the leading path for synthesis and the rest being δ and ε are the basic polymerase for the lagging synthesis of strand.

Mostly the enzyme that are present inside the cell for the help of getting the smooth functioning of the RNA is mostly for the purpose off good function of all the chemical reactions. RNA polymerase enzyme helps to synthesize RNA by pointing a strand of DNA. This enzyme is also the enzyme that plays the role of copying DNA and its sequence into an RNA strand at the time of transcription.

RNA replication in eukaryotes takes place in the nucleus. Some RNA molecules play important roles in catalyzing biological processes within cells and regulating gene expression. It also helps to activate cellular signals by transmitting multiple responses and recognizing them. One of the techniques its miles concerned in is protein synthesis and additionally getting the ribosomes protein synthesized.

RNA replication enzymes in prokaryotes

Prokaryotes are the organism that have only one cell and cannot be seen without microscope. They do not have a nucleus unlike the eukaryotes.

Inside the prokaryotes, the origin for the point of replication to take place is only one in number and takes place in two of the opposing directions at the similar tome and is seen to occur inside the cytoplasm o the cell.

On the other if the eukaryotes are taken into consideration, they have many site for getting the process of replication conducted and with not being concerned to two paths. The process is made to be useful in several ways and is unidirectional at the part of replication inside the cell within the nucleus.

Inside the prokaryotes there are several ways to get done with the process of the DNA to get it replicated. They are dispersive, semi-conservative and conservative. The very common one to be described is the conservative one that says that the original DNA stays linked and the new made strands of it called the daughter strands are also made to connect.

Image credit-

The prokaryotes are divided only into two domains. One is said to be the bacteria and the other is archaea. The bacteria are said to be common and the one known widely for the cells of prokaryotes. They lack the entire inside membrane and have flagella for moving. The oldest known prokaryote is 3.5 billion years old.

Both of the type of organism found being eukaryotes and the prokaryotes use an enzyme that is common to both and that is the RNA polymerase which is used to transcribe the RNA from DNA. The prokaryotes use up only one RNA polymerase for all the types of RNA or transcription. They do have only one site for replication but has several sequences for getting it repeated in form of array.

RNA replication enzymes in bacteria

Bacteria are said to be the organism that live freely and are single cell also regard to as the prokaryotes. They are not much longer and can be seen at any place

The microbes of RNA have themselves replicated via either of the two ways. The first being the process of the RNA being dependent on the synthesis of RNA and the other being the process of reverse transcription that is the RNA dependent on synthesis of DNA and is followed by transcription.

Inside the bacteria, all the transcription that Is seen is by a single and special type of RNA polymerase. This polymerase has with it four of its varieties and catalytic units with only one regulatory unit called as the sigma. They only have one copy site but many strings to make it repeat in a loop. The manner is made to be beneficial in numerous methods and is unidirectional on the a part of replication within the mobileular in the nucleus.

Some bacteria are harmful, but most serve a useful purpose. They support many forms of life, both plant and animal, and they are used in industrial and medicinal processes. Bacteria are like eukaryotic cells in that they have cytoplasm, ribosomes, and a plasma membrane. Features that distinguish a bacterial cell from a eukaryotic cell include the circular DNA of the nucleoid, the lack of membrane-bound organelles.

RNA-dependent RNA polymerase or RNA replicase is an enzyme that catalyzes the replication of RNA from an RNA template. DNA replication initiates at specific points, called the origins, where the DNA double helix is unwound. A short segment of RNA, called a primer, is then synthesized and acts as a starting point for new DNA synthesis. An enzyme called DNA polymerase next begins replicating the DNA by matching bases to the original strand.


I am Ankita Chattopadhyay from Kharagpur. I have completed my B. Tech in Biotechnology from Amity University Kolkata. I am a Subject Matter Expert in Biotechnology. I have been keen in writing articles and also interested in Literature with having my writing published in a Biotech website and a book respectively. Along with these, I am also a Hodophile, a Cinephile and a foodie.

Recent Posts